Trichomonas vaginalis, Nucleic Acid Amplification, Varies

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Trichomonas vaginalis Amplified RNA detects T. vaginalis using Transcription-Mediated Amplification. Aliases: Trichomonas RNA, T. Vag, Trich.

Trichomonas vaginalis, Nucleic Acid Amplification, Varies

OVERVIEW

The Trichomonas vaginalis Nucleic Acid Amplification test is crucial for detecting Trichomonas vaginalis in specimens like urine, cervical/endocervical, or vaginal samples. Utilizing Transcription-Mediated Amplification to amplify nucleic acids, it ensures accurate identification of the parasite. Reported as Trichomonas vaginalis Amplified RNA, it is also known as Trichomonas RNA, T. Vag, and Trich, playing a key role in diagnosing and managing Trichomoniasis for timely treatment.

 


 

METHOD NAME

Transcription-Mediated Amplification

 

AVAILABILITY

TBC

 

REPORTING NAME

Trichomonas vaginalis Amplified RNA


ALIASES

Trichomonas RNA

T. Vag

Trich

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SPECIMEN TYPE 

Varies

 

SHIPPING INSTRUCTIONS

TBC

 

SPECIMEN REQUIRED

Submit only 1 of the following specimens:

 

Specimen Type: Endocervix/Cervix

Supplies: Aptima Unisex Swab Collection Kit 

Container/Tube: Aptima Unisex Swab

Specimen Volume: Swab

Collection Instructions:

  1. Specimens must be collected using the Aptima Unisex Swab Collection Kit.
  2. Use cleaning swab (white shaft) to remove excess mucus from endocervix/cervix.

Discard the cleaning swab.

  1. Insert second swab (blue shaft) 1 to 1.5 cm into endocervical canal, and rotate swab gently for 30 seconds. Avoid touching vaginal wall when removing swab.
  2. Place second swab (blue shaft) into Aptima transport tube provided in collection kit. Snap off swab at score line so swab fits into closed tube.
  3. Cap tube securely and label tube with patient's entire name and collection date and time.
  4. Maintain swab container at 2 to 30 degrees C (refrigerate temperature is preferred), transport within 60 days of collection.

 

Specimen Type: Vaginal

Supplies: Aptima Multitest Swab Collection Kit 

Container/Tube: Aptima Multitest Swab

Specimen Volume: Swab

Collection Instructions:

  1. Specimens must be collected using the Aptima Multitest Swab Collection Kit.
  2. Insert swab (pink shaft) about 5 cm past introitus and rotate gently for 30 seconds.
  3. Place swab into Aptima transport tube provided in collection kit. Snap off swab at score line so swab fits into closed tube.
  4. Cap tube securely and label tube with patient's entire name and collection date and time.
  5. Maintain swab container at 2 to 30 degrees C (refrigerate temperature is preferred), transport within 60 days of collection.

 

Specimen Type: ThinPrep Specimen (Endocervix/Cervix)

Supplies: Aptima Thin Prep Transport Tube 

Container/Tube: Thin Prep Transport Tube

Specimen Volume: 1 mL

Collection Instructions:

  1. Collect ThinPrep sample as per normal collection process.
  2. ThinPrep specimen must be aliquoted (as outlined below) before it is processed/tested for Pap smear.
  3. Vortex ThinPrep/PreservCyt vial 3 to 10 seconds. Within 1 minute of vortexing:
  4. Transfer 1 mL of specimen into the Aptima specimen transfer tube using a disposable transfer pipette or a pipette tip containing a filter (aerosol barrier or hydrophobic plug).
  5. Process only 1 ThinPrep and transfer tube set at a time.
  6. Recap Aptima specimen transfer tube tightly and gently invert 3 times to mix.
  7. Label Aptima transfer tube with appropriate label.
  8. Use remainder of ThinPrep specimen for Pap testing.
  9. Maintain specimen transport tube at 2 to 30 degrees C (refrigerate temperature is preferred), transport within 30 days of collection when stored at 2 to 14 degrees C or within 14 days when stored at 15 to 30 degrees C.

 

Specimen Type: Urine

Supplies: Aptima Urine Transport Tube 

Container/Tube: Aptima urine specimen transport tube

Specimen Volume: 15 to 20 mL

Collection Instructions:

  1. Patient should not have urinated for at least 1 hour prior to specimen collection.
  2. Patient should collect first portion of random voided urine (first part of stream) into a sterile, plastic, preservative-free container.
  3. Within 24 hours of collection, transfer 2 mL of urine into the Aptima urine specimen transport tube using the disposable pipette provided. The correct volume of urine has been added when the fluid level is between the black fill lines on the Aptima urine transport tube.

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